detection of babesia ovis using polymerase chain reaction.

Detection of Babesia ovis Using Polymerase Chain Reaction.

Detection of Babesia ovis Using Polymerase Chain Reaction

A procedure was developed for delection of Babesia ovis (B.ovis) infection in blood samples containing infected erythrocytes by polymerase chain reaction (l'CR). In order to deteet the DNA parasite in blood sample two sets of oligonucleotides were designed according to the nucleotide sequences of the l8S rRNA and ATP-binding prote in genes of H ovis. These genes were amplified at 286 and 453bp ...

Detection of Leishmania DNA in paraffin embedded specimens of chronic lupoid leishmaniasis using polymerase chain reaction

Background: Chronic lupoid leishmaniasis (CLL) is a chronicform of cutaneous leishmaniasis that is usually resistant to antileishmania agents and leishmania is not seen in the histologicsections. We used polymerase chain reaction (PCR) to detectleishmania DNA in CLL specimens.Method: This descriptive cross sectional study was done on20 paraffin embedded specimens of CLL cases referred to theder...

Detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction

Five common tetracycline resistance genes tet(A), tet(B), tet(M), tet(O) and tet(S) were studied by polymerase chain reaction in 100 bacteria isolated from Iranian fish farms. In the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. Nine isolates out of 46 Aeromonas spp. contained eithe...

Actinobaculum suis Detection Using Polymerase Chain Reaction

Actinobaculum suis is an important agent related to urinary infection in swine females. Due to its fastidious growth characteristics, the isolation of this anaerobic bacterium is difficult, thus impairing the estimation of its prevalence. The purpose of this study was to develop and test a polymerase chain reaction (PCR) for the detection and identification of A. suis and then compare these res...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction